Bacteriological analyser hyborg Dx RED2

HYBORG – AUTOMATED PROCESSING AND REPORTING

The hyborg processes hybcell assays in a fully automated format. It is tailored for operation in a (clinical) routine laboratory and guarantees limited hands-on time, ease of usage, and clear reports.

 

The test is based on three steps: sample preparation and DNA purification, PCR and identification with the hybcell.

 

The PCR screening for bacteria (16S rDNA) and fungi (28S rDNA) and the follow up identification process on the hybcell are termed compact sequencing, and the test components (PCR mixes and hybcells) and processes are the same for all intended uses. The treatment of the different sample matrices differs strongly between analysing whole blood for sepsis diagnostics or BAL / positive blood cultures for diagnosing pneumonia and other infections.

 

Whereas analysing whole blood requires the removal of human DNA using the GINA kit, BAL or positive blood cultures require no DNA extraction process whatsoever and use the modulation buffer LINA instead.

 

 

DIAGNOSING SEPSIS – WITHOUT CULTURING / FROM BLOOD

CubeDx supports clinicians by providing a very early indication of sepsis-causing microorganisms directly in whole blood. Testing whole blood reduces the time to result to just 3 hours! Our multiplex technology allows for identification of more than 100 targets (bacteria, fungi and resistance genes) in parallel.

 

 

SIMPLE WORKFLOW

Fresh EDTA blood (0.5ml) serves as a sample. To validate a negative result, an Internal Process Control (IPC) can optionally be introduced into the sample.

 

During the GINA process, most cells of human origin are depleted before DNA is purified.

The enriched microbial DNA is amplified using a choice of PCR master-mixes: pan-bacterial PCR, pan-fungal PCR or PCR to screen for several resistance genes.

 

Positive samples are transferred into the hybcell cartridges, and the microorganisms and resistance genes are identified by the hybcell and its compact sequencing process, with the results subsequently presented in a comprehensive report.

 

FEATURES / ADVANTAGES:

• Results in just 3 hours!

• Yield of up to 98% of microbial DNA

• Limit of detection (whole blood): 20 CFU/mL

• Early and comprehensive results from 0.5 mL whole blood

• Sensitive and parallel identification of bacteria, fungi, and resistance genes

• Identification of multiple pathogens/mixed infections

• Simple handling, an optimized workflow with 24/7 availability and immediate results