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- Cell sorter Aurora CS
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Details
The Cytek Aurora CS system was built with leveraging the power of Full Spectrum Profiling technology in a flexible and easy-to-use platform that meets the diverse needs of your laboratory.
The Cytek Aurora CS system provides remarkable flexibility, enabling the use of a wide array of new fluorochrome combinations without requiring the reconfiguration of the detectors. Sharing the same optical system as the Cytek Aurora analyzer allows for the easy transfer of experiments from the analyzer to the Aurora CS system without the need to modify your panels. The state-of-the-art optics and low-noise electronics ensure high resolution and high sensitivity for identifying and isolating rare cell populations deep into the gating hierarchy.
The result is a system that delivers exceptional performance at the single-cell level which can resolve the most challenging cell populations, such as cells with high autofluorescence or low expression of key biomarkers, regardless of assay complexity. Equipped with advanced sorting features and monitoring tools, the Cytek Aurora CS system can isolate viable and functional cells for downstream studies.
So Many Colors
Up to 40 colors demonstrated including fluorochromes with emission spectra in close proximity to each other.
Exceptional Sensitivity
Sensitivity redefined using state of the art optics and low noise electronics.
A New Level of Flexibility
No changing optical filters for any fluorochrome. Use any commercially available fluorochrome excited by the onboard lasers.
Seamless Sorting
Transfer assays with ease, experience automated drop delay, clog detection, sort monitoring, and sort reporting.
In-Depth Sorting Functionality
The Cytek Aurora™ CS system combines FSP™ technology and high-end sorting functionality to dig deep into cellular assays. Sort populations up to 64 levels deep into the gating hierarchy, up to two-way sorting with 15 mL tubes, sort up to 6 ways into 1.5 mL or 5 mL tubes, or sort into 384-well or 96-well plates with index sorting capability. Live view camera monitoring helps monitor side-stream position, and other built-in automated tools ensure successful sorting.
Autofluorescence Extraction
The Full Spectrum Profiling technology employed by the Aurora CS system can improve data clarity and resolution with autofluorescence extraction. Certain sample types, such as yeast and tumor samples, present difficulty in resolving due to their highly autofluorescent nature. For applications involving highly autofluorescent particles, the SpectroFlo® CS software autofluorescence extraction tool achieves new levels of resolution and increases sorting performance of challenging samples.
Characteristics
| OPTICAL PLATFORM | Aurora CS system contains a fixed optical assembly with the capacity to be configured with up to five spatially separated laser beams. Laser delays are automatically adjusted during instrument QC |
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| BEAM GEOMETRY | Flat-top laser beam profile with narrow vertical beam height optimized for small particle detection |
| STANDARD OPTICAL CONFIGURATION | ▪ Violet detector module: 16 channels unevenly spaced bandwidth from 420-829 nm ▪ Blue detector module: 14 channels unevenly spaced bandwidth from 498-829 nm ▪ Red detector module: 8 channels unevenly spaced bandwidth from 652-829 nm |
| FLUIDIC MODES | Fluidics startup, fluidics shutdown, SIT flush, purge filter, clean flow cell, aseptic clean, sample return |
| FLUIDIC RESERVOIRS | 10 L sheath and waste fluid containers with level-sensing provided. 3 L cleaning tank also included |
| FLUORESCENCE LINEARITY | FITC R2 ≥0.995 / PE R2 ≥0.995 |
| SORT COLLECTION | Up to 2-way sorting: 15 mL polystyrene and polypropylene tubes. Up to 6-way sorting: 5 mL and 1.5 mL polystyrene and polypropylene tubes 96-well and 384-well plates with index sorting. Custom plate options available. |
| SORT MODES | Multiple optimized sort modes for purity, enrichment, mixed, and single cell plus user definable sort modes. Deposit 1 cell per well into 96 wells in less than 2 minutes and 384 wells in less than 5 minutes. |
| LASERS | ▪ 355 nm: 20 mW, ▪ 405 nm: 100 mW, ▪ 488 nm: 50 mW, ▪ 561 nm: 50 mW, and ▪ 640 nm: 80 mW |
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| FORWARD AND SIDE SCATTER DETECTION | FSC: high-performance semiconductor detector with 488 nm bandpass filter. SSC: two high-performance semiconductor detectors with 405 nm and 488 nm bandpass filters |
| SAMPLE FLOW RATES | Adjustable in increments of 7 µL/min from 10 µL/min to 80 µL/min |
| SAMPLE INPUT FORMATS | 12 x 75 mm or 15 mL polystyrene or polypropylene tube with sample mixing |
| FLUORESCENCE SENSITIVITY | ▪ FITC: ≤5 MESF ▪ PE: ≤4 MESF ▪ APC: ≤3 MESF ▪ Pacific Blue: ≤4 MESF |
| DATA ACQUISITION RATE | 25,000 events/s (five laser system) |
| NOZZLES | Quick-replace 70, 85, 100, and 130 µm nozzles with optimized and user definable pressure and sorter settings. Up to 6-way sorting with any nozzle size. |
| SPECTROFLO® CS SOFTWARE | Live unmixing during acquisition and sorting. Sort on raw or unmixed data Developed specifically to streamline assay setup, data acquisition, and file export. Automated QC module. Autofluorescence extraction. Manual and automated drop delay functionality. Default and customizable sort modes and nozzle settings. Sort collection tube volume monitoring and live view. Autogenerated sort reports. |
Service and Warranty
We provide premium service and maintenance for every device and consumable:
• Comprehensive maintenance of medical equipment
• Installations, commissioning
• Warranty and post-warranty service
• Instruction, training and consultations for your team
More details on the SERVICE page
Contacts for communication:
| Contact phone | +38 (067) 120 95 75 |
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| E-mail: | service@empirica.healthcare |