Hypoxic H Worksations

Don Whitley Hypoxic Workstations: Continuous Control of In Vivo Conditions for Cell Cultures

To replicate physiological normoxia and study hypoxia, traditional CO₂ incubators often prove insufficient. Moving samples for analysis or media changes inevitably leads to gas fluctuations, causing cellular stress and compromising experimental reproducibility.

 

The standard Don Whitley H-Series hypoxic workstations solve this problem. They create a continuous, isolated environment, allowing researchers to seed, passage, and perform microscopy on cells without any disruption to the set parameters. This is critical equipment for modern research in oncology, neurology, stem cells, and cardiovascular pathology.

 

Key Benefits for Your Laboratory

• Zero Cellular Stress: Guaranteed stability of the gas environment, temperature (up to 45°C), and humidity (up to 80%) during all manipulations.
• Ultra-Precise O₂ & CO₂ Control: Oxygen and carbon dioxide regulation in 0.1% increments. The internal sensor is calibrated in real-time directly within the incubation zone, reflecting the actual conditions the cells experience.
• Operational Cost Optimization: Well-designed airlocks ensure fast loading of materials with minimal consumption of expensive gas mixtures.
• Maximum Ergonomics and Convenience: Patented oval glove ports provide freedom of movement and allow the operator to work bare-handed, maintaining ideal tactility for complex manipulations with pipettes and flasks.
• Data Digitalization: Color touchscreen interface with data logging capabilities for a complete audit of experimental conditions.

 

Model Selection: From Basic Tasks to Process Scaling

A wide range of standard workstations allows you to choose the ideal solution based on your laboratory space, sample volume, and the need to use additional equipment inside the chamber.

 

Model	Chamber Volume	Airlock Features	Additional information
H25	180 L	6 L (20-second cycle)	The best choice for personal projects and laboratories with limited space. The ultra-fast airlock saves time and gas.
H35	300 L	12 L (holds 40 Petri dishes)	The optimal balance between capacity and footprint. The perfect "workhorse" for most fundamental research.
H45	439 L	2 L (fast cycle)	Three access ports and an increased chamber width comfortably accommodate small additional equipment (e.g., a centrifuge or vortex).
H55	600 L	Two independent 12 L airlocks	Four ports and two airlocks allow two operators to work simultaneously without interfering with each other. An excellent solution for busy departments.
H85	300 L	36 L (Large Capacity)	Specifically designed to accommodate large instruments, such as microscopes or Agilent Seahorse analyzers. A unique cooling option down to 8°C is available.
H95	600 L	36 L (Large Capacity)	The largest chamber and airlock capacity in the standard lineup. Allows loading up to 133 microplates in a single cycle and accommodates bulky apparatus.