NGS Kit for Detection of Germline and Somatic Mutations — truNGS® BRCA Plus Kit

This product is a complete NGS solution for the detection of germline and somatic variants (SNVs and Indels) in the BRCA1, BRCA2 and STK11 genes from nucleic acids extracted from blood, fresh frozen or formalin-fixed paraffin-embedded (FFPE) tissue samples.

 

The kit is designed to detect SNVs and Indels in the BRCA1, BRCA2 and STK11 genes from nucleic acids isolated from blood, fresh frozen or FFPE tissue specimens.

 

The protocol consists of two main steps:

 

(A) Library preparation (input DNA fragmentation, adapter ligation, PCR amplification of individual libraries);

(B) Hybrid capture and target enrichment (library pooling, probe hybridization, capture and post-capture PCR amplification).

 

For use on Illumina and MGI platforms only.