Kit for detection of Acanthamoeba T4

Kit for detection of Acanthamoeba T4 is a real-time PCR test and detects the 18S rRNA gene of Acanthamoeba species of genotype T4, which causes approx. 86% of the Acanthamoeba keratitis cases worldwide.

 

Acanthamoeba keratitis (AK) is a severe sight-threatening eye infection, and an early diagnosis offers the best chance of a cure.

 

Product features

 

• 100% specificity for Acanthamoeba species of genotype T4 (A. castellani, A. lugdunensis, A. mauritaniensis, A. polyphaga, A. rhysodes, A. royreba)

 

• Correct detection of Acanthamoeba T4 species

 

• Enables specific Acanthamoeba keratitis therapy

 

• Clinically validated for ocular, corneal, and lens samples associated with keratitis

 

Kit contents

 

• Primers and probes for detection of Acanthamoeba T4 (FAM) and internal positive control DNA (Cy5)

 

• DNA target for IPC

 

• Positive control for Acanthamoeba T4

 

• DNA reaction mix

 

• Nuclease-free water

 

Key Benefits 

 

• Optimized PCR to handle inhibitors

 

• UNG in reaction mix to eliminate amplicon carryover

 

• Rapid hot-start Taq DNA polymerase reduces run time

 

• ROX dye as passive reference

 

• Internal Positive Control to avoid false negatives

 

• Compatible with a wide range of real-time PCR instruments (Applied Biosystems, Roche, Bio-Rad, Tetracore, Analytik Jena)

 

• Fast and reliable results in <2 hours from extraction