NGS Endometrial Cancer Panel Kit for Genetic Marker Detection – truNGS®

This product is designed to detect SNVs, Indels, and CNVs in 31 marker genes and hotspots in 6 pharmacogenomics genes associated with endometrial cancer from nucleic acid extracted from fresh frozen/formalin-fixed paraffin-embedded (FFPE) tissue.

 

The protocol involves two major steps:

 

A. Library Preparation: input DNA fragmentation, adapter ligation, PCR amplification of individual libraries.

 

B. Hybrid Capture and Target Enrichment: library pooling, probe hybridization, capture, and post-capture PCR amplification.

 

For use on Illumina and MGI platforms only.