TRUPCR® KRAS Real-time PCR Kit

The TRUPCR® KRAS PCR Kit (K-RAS Kit) is an in vitro diagnostic test intended for the qualitative detection of KRAS somatic mutations in genomic DNA extracted from fresh, frozen or formalin-fixed paraffin-embedded (FFPE) tissue.

 

The TRUPCR® KRAS PCR Kit is based on allele-specific amplification and is achieved by ARMS PCR. Taq DNA polymerase is extremely effective at distinguishing between a match and a mismatch at the 3’-end of a PCR primer. The TRUPCR® KRAS Real-time PCR Kit is designed to selectively amplify mutant-specific sequences in samples that contain a mixture of wild-type and mutated DNA. The most common mutations are found in codons 12, 13 and 61. The detection is achieved using fluorescent probes labelled with FAM and HEX. The TRUPCR® KRAS PCR Kit is composed of 11 assays for the detection of the KRAS mutations and a reference control gene of KRAS region without any known polymorphism / mutation.

 

Key Features of the TRUPCR® KRAS Real-time PCR Kit:

• Selective amplification of DNA containing mutation with ARMS Technology.

• Sensitive to detect up to 1% mutation in KRAS gene.

• Detects 22 different mutations in a single run.

• Extraction control included to avoid false-negative results.

• Rapid, more reliable, comprehensive and cost effective tests.

• Easy work flow & compatible with various Real Time PCR instruments.

 

Mutations	Contents
Ex 2, c12	G12C (c.34G>T), G12S (c.34G>A), G12R (c.34G>C), G12V (c.35G>T), G12D (c.35G>A)
Ex 2, c13	G13D (c.38G>A)
Ex 3, c59	9 A59T (c.175G>A), A59E (c.176 C>A), A59G (c. 176 C>G)
Ex 3, c61	Q61K (c.181C>A), Q61L (c.182A>T), Q61R (c.182A>G), Q61H (c.183A>T), Q61H (c.183A>C)
Ex 4, c117	K117E (c.349A>G), K117R (c.350A>G), K117N (c.351A>C), K117N (c.351A>T)
Ex 4, c146	A146T (c.436G>A), A146P (c.436G>C), A146V (c.437C>T)

 

3B1295 – 24 tests

3B1261 – 48 tests