Reagents for flow cytometry

Flow cytometry is a modern analytical method used to study the physical, chemical, and biological characteristics of cells or other particles in suspension. The principle of flow cytometry is based on the analysis of individual particles that pass through a narrow channel along with a liquid known as an isotonic solution. During this process, the particles are exposed to laser light, and their scattering and emission are analyzed to obtain information about size, structure, and biological markers.

 

Flow cytometry is widely used in clinical diagnostics, molecular biology, immunology, and other sciences. Its main areas are the study of cell surface phenotype, determination of immune status, cytotoxicity analysis, and the detection of cell subtypes such as T lymphocytes, B lymphocytes, leukocytes, monocytes, and T regulatory cells.

 

Types of flow cytometry reagents

 

Flow cytometry reagents are a key component in performing analyses. They ensure the accuracy and reliability of research. Main types of reagents:

• Fluorophores and dyes. These products are used to label specific biomolecules in cells. A fluorophore is a molecule that emits light in response to laser irradiation, allowing the visualization of antibodies bound to cell markers.
• Labeled antibodies. Used to identify cell subtypes and detect specific molecules on or inside cells.
• Isotonic solutions. These reagents ensure the stability of cells in suspension and the correct operation of the cytometer.
• Compensation control sets. Used to avoid errors associated with the unmixing of data from different fluorophores.
• DNA and RNA isolation reagents. Used to analyze the cell cycle, apoptosis, or other processes related to genetic information.

 

How to choose reagents for flow cytometry

 

The selection of reagents for flow cytometry depends on the purpose of the study and the characteristics of the sample. Key aspects of the choice:

1. The purpose of the study. For example, for immunogram analysis, specific markers should be used that will allow the detection of T cells, B cells, and other subtypes.
2. Fluorophore compatibility. Avoid overlapping emission spectra by using proper compensation.
3. Product quality. Choose reagents from proven manufacturers to avoid errors in results.
4. Sample type. For blood analysis, for example, it is important to use reagents to label leukocytes, lymphocytes, or other cell types.

 

Sample preparation tips

 

The quality of sample preparation directly affects cytometry results. Follow these recommendations:

• Use isotonic solutions to preserve cellular integrity.
• Remove debris (cell debris) to increase analysis accuracy.
• Use control kits to ensure correct results.
• Perform immunocytochemical staining according to the reagent manufacturer's protocol.

 

Where to buy flow cytometry reagents?

 

We recommend contacting the Empirica online store to purchase high-quality flow cytometry reagents. This company offers a wide range of products for flow cytometry, including antibodies, fluorophores, isotonic solutions and control kits.

 

Our online store provides fast delivery of equipment in Kiev and throughout Ukraine. They offer convenient ordering conditions, expert advice and a guarantee of product quality. The correct selection and use of cytometry reagents will ensure high quality results and allow you to obtain valuable information about biological processes.